Thesis Abstract and Dissertation Abstracts
Direct Organogenesis Of Heliotropium Crassavicum L. Using Stem Explants And Their Free Radical Scavenging Effect
Abstract Category: Science
Course / Degree: M.Sc.
Institution / University: Annamalai University, India
Published in: 2012
Heliotropium curassavicum L. is a sand binder salt marsh herb with enormous traditional value and widely found in South Asia America and Europe. In the direct method of regeneration from stem explants, we observed the maximum number of shoot regeneration after four weeks culture of MS elongation medium with 2.0 mg/L of 2, 4-D (17.27±0.51). It was clear that MS medium with 2.0 mg/ml 2, 4-D alone suitable for shoot multiplication as well as shoot elongation then compared to other combination of auxin and cytokinin. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing 2, 4-dichlorophenoxy acetic acid (0.5-3.0 mg/L).
The maximum number of root regeneration (6.4±0.416) and root length (6.08±0.07) were observed in MS rooting medium fortified with 2.5 mg/L of 2, 4-D after 2 weeks of culture. 85% of invitro raised plantlets with well-developed shoots and roots were transferred to exvivo conditions into polythene bag containing sterile compost with ratio (v/v/v) of organic fertilizer: sand: peat (1:2:2; 3:1:0 or 2:2:1). Sixty five percent of acclimated plants were transferred to the pots under full sun where they grew well without any detectable phenotypic variations. Invitro raised plantlet methanolic extract showed better DPPH radical scavenging activity of about 89% at 10ppm. The growing interest in the substitution of antioxidants with natural ones has fostered research on plant sources and screening of raw materials to identify new antioxidants.
Report Keywords/Search Tags:
direct method; 2,4-D: sterile compost; stem; salt marsh
This Report Abstract may be cited as follows:
V.Dheepak, K.Satyavani, S.Gurudeeban, and T.Ramanathan, 2012. Direct organogenesis of Heliotropium crassavicum L. Using stem explants and their free radical scavenging effect
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Heliotropium Crassavicum (click to enlarge) |
Submission Details: Report Abstract submitted by Gurudeeban Selvaraj from India on 19-Feb-2013 08:43.
Abstract has been viewed 2646 times (since 7 Mar 2010).
Gurudeeban Selvaraj Contact Details: Email: gurudeeb99@gmail.com
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