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Development Of An Efficient In Vitro Micropropagation Protocol For Medicinally Important Plants Achyranthes Aspera L. And Achyranthes Bidentata Blume  


Abstract Category: Science
Course / Degree: Master of Science Degree
Institution / University: University of Chittagong, Bangladesh
Published in: 2012


Paper Abstract / Summary:

Medicinal plants are the great natural resources but due to lack of knowledge, arbitrary use and lack of conservation measures many important medicinal plant species are becoming extinct, endangered and threatened. The present study attempted to develop in vitro micropropagation protocol for two important medicinal plants of Bangladesh Achyranthes aspera and Achyranthes bidentata. Shoot tip and nodal segment of healthy plants from natural condition were used as explant for direct organogenesis. Varying concentrations of auxins and cytokinins both alone and in combination were used. For A. aspera, shoot tip produced the best shoot multiplication (12.2 shoot/explant) in hormonal combination of 2.0 mgl-1 BAP + 0.5 mgl-1 NAA while nodal segment and single cytokinin media showed poor response. But for A. bidentata, nodal segment explant produced best response in single cytokinin media (5.0 mgl-1 of BAP) in terms of maximum number of shoots (10.5). For root induction and root development, IBA in full strength MS media was proved better. However, A. bidentata produced better rooting response than A. aspera. Acclimatization of these in vitro grown plantlets in natural soils was also promising which was 70% for A. aspera and 80 % for A. bidentata, respectively.


Paper Keywords/Search Tags:
In vitro, micropropagation, Achyranthes aspera, Achyranthes bidentata, shoot tip, nodal segment.

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Paper Images:
Science - Development Of An Efficient In Vitro Micropropagation Protocol For Medicinally Important Plants Achyranthes Aspera L. And Achyranthes Bidentata Blume Multiple shoot development from A. bidentata nodal segment cultured in vitro
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Submission Details: Paper Abstract submitted by MD JAKIR HOSSAIN from Bangladesh on 16-Mar-2013 18:11.
Abstract has been viewed 3171 times (since 7 Mar 2010).

MD JAKIR HOSSAIN Contact Details: Email: jakirgeb2@yahoo.com Phone: 01753937280



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