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Genotypic Characterization And Sequencing Of Enterotoxins From B. Thuringiensis Strains Isolated On Amazon State, Brazil  


Abstract Category: Science
Course / Degree: Biotechnology/MasterĀ“s
Institution / University: Universidade Federal do Amazonas, Brazil
Published in: 2009


Dissertation Abstract / Summary:

Bacillus thuringiensis is a Gram-positive bacterium commonly used in the tropical disease vectors and agriculture pragues control. Despite of its use both agriculture and human health, this bacterium can be enterotoxins producer that are also present in a few Bacillus cereus strains, emphasizing the non-haemolytic enterotoxin (NHE), haemolysin BL (HBL) and enterotoxin T (BceT) that have been related to food poisoning outbreaks reported in the literature. Thereby, this work had as a purpose to identify and to realize a genotypic characterization of these enterotoxins in one hundred B. thuringiensis strains isolated in the Amazon State, as well as to achieve the sequencing of these enterotoxin genes starting from the product of Polymerase Chain Reaction (PCR). The prevalence of these enterotoxin genes in B. thuringiensis strains by PCR method was relatively high, of which the results for the seven genes researched (bceT, hblA, hblD, hblC, nheA, nheB and nheC) showed different between themselves. By the genotypic profile were determined 27 groups and was evidenciated that 41% of the strains were positives for all the enterotoxin genes, whereas 3% were negatives for all the genes studied.. The analysis of the nucleotides and amino acids sequences of the Amazonian B. thuringiensis strains identified similarities with the nucleotides and amino acids sequences that are deposited in the GenBank and EMBL databases.


Dissertation Keywords/Search Tags:
Bacillus thuringiensis, Bacillus cereus, enterotoxins, genotypic profiles

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Submission Details: Dissertation Abstract submitted by Marcos Pessoa from Brazil on 17-Oct-2015 07:40.
Abstract has been viewed 1688 times (since 7 Mar 2010).

Marcos Pessoa Contact Details: Email: mrpessoa@outlook.com



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